Antibodies Essay Example | Topics and Well Written Essays - 1500 words

Antibodies - Essay ExampleIt is used in the manufacture of unsaturated polyester resins and is used as solvent for surfactants, paints and cleaning agents1. It is also used as antifreeze for de-icing aircrafts at airports as it lowers the freezing point of water. Recent applications include use as a humectant and a preservative. It is used in cosmetics, pharmaceutical preparations and also in electronic cigars2. The structure of 1,2-Propanediol comprises of three carbons with a stereogenic center at the central carbon3. It exists in two racemic forms S and R as shown in figure 1 below. mannequin 1 Chemical structure (S and R form) of 1,2-propanediol 3 1,2-Propanediol as well as 1,3-Propanediol stack be manufactured though bioengineered microorganisms. By turning on and off some genes, it is possible to make the microorganism overproduce a specific metabolic product. As shown in figure 2, the intermediate metabolism of Escherichia coli can be shifted to produce 1,2 and 1,3-propanedi ol using enzymes such as aldose reductase from rat lens, E. coli glycerol dehydrogenase and Klebsiella pneumoniae glycerol dehydrogenase and 1,3-propanedioloxidoreductase4. There are various biochemical pathways through which 1,2-propanediol can be produced. Figure 2 Metabolic pathway engineering of E. ... It was shown that E. coli can grow on the L-fucose and L-rhamnose deoxy sugars as sole carbon and energy sources resulting in the production of propanediol. The low gear instance of metabolic engineering to produce 1,2-propanediol was when Altaras and Cameron metabolically bioengineered the 1,2-propanediol pathway in E. coli7. In 1999, they reported that E. coli that overexpressed methylglyoxal synthase gene produced 1,2-propanediol. Expression of methylglyoxal synthase or glycerol dehydrogenase led to anaerobiotic production of around 0.25 g of 1,2-propanediol per liter. The yield was found to be higher both the enzymes were coexpressed7. The development After their first repor t of the production of 1,2-propanediol from metabolically engineering E. coli, Altaras and Cameron again in 2000 published another report. This time they reported enhanced production of 1,2-propanediol8. They investigated three methods. First method involved the elimination of lactate byproduct. The stand by method involved the construction of a complete pathway from dihydroxyacetone phosphate an intermediate of the glycolytic cycle. The third method involved bioprocessing improvements through fed-batch fermentation using the scoop bioengineered strains. They were able to successfully produced a final yield of 0.19 g of 1,2-propanediol per gram of consumed glucose. Many later studies have investigated the production of 1,2-propanediol by metabolically engineered bacteria. Berrios-Rivera, San and Bennett studied the do of various cofactor manipulations on the production of 1,2-propanediol9. They used sugars that were similar to glucose and that can be fed into glycolysis for pyru vate production. The sugars used had different oxidation